| 產品編號 | bsm-33004M-HRP |
| 英文名稱 | Mouse Anti-His tag/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的聚組氨酸抗體標簽單克隆抗體 |
| 別 名 | HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
| 規(guī)格價格 | 100ul/1200元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 免疫學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 6E6 |
| 交叉反應 | Human, |
| 產品應用 | WB=1:10000-1:20000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated HHHHHH peptide |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 儲 存 液 | Constituents: 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin, 50% glycerol. Or Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin. Reconstitute with sterile distilled water. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 蛋白質純化的新途徑─應用His-Tag抗體檢測His-Tag融合蛋白 1） 6xHis與不同的Sepharose純化填料結合（捆綁），可不受構象的影響，在天然或變形的條件下可進行進一步純化。 2） 可在溫和的洗脫液環(huán)境中，其結合、洗滌及洗脫步驟不會對蛋白結構產生影響。 3） 6xHis標簽比普通（Tag）要小得多，經純化后的蛋白可直接進行下游操作?？捎糜谌魏伪磉_系統(tǒng)（包括： 細菌，桿狀病毒和哺乳動物） 4） 6xHis標簽在生理溶液pH下不帶電荷，其標簽不會干涉重組蛋白的結構和功能， 6xHis不會影響蛋白分泌。 5） 6xHis標簽免疫原性差，重組蛋白可以不必除去標簽而直接作為抗原進行免疫。 |
| 產品圖片 |
50 ng rGFP-His protein (bs-33009P) per lane probed with His tag monoclonal antibody respectively, HRP conjugated (bsm-33004M-HRP) at 1:100000 dilution and 4°C overnight incubation.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
