| 產(chǎn)品編號 | bs-5968R-FITC |
| 英文名稱 | Rabbit Anti-NARG2/FITC Conjugated antibody |
| 中文名稱 | FITC標記的谷氨酸受體調(diào)節(jié)蛋白2抗體 |
| 別 名 | BRCC1; NARG 2; NARG2_HUMAN; NMDA receptor regulated protein 2; NMDA receptor-regulated protein 2. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 細胞生物 免疫學 染色質(zhì)和核信號 信號轉(zhuǎn)導 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Rat, (predicted: Mouse, Dog, ) |
| 產(chǎn)品應用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 110kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NARG2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: NARG2 is expressed at relatively high levels in dividing and immature cells, and is down-regulated upon terminal differentiation. NARG2 is a novel (S/T)PXX motif-containing nuclear protein; this motif is present in many transcription factors as well as other regulatory proteins that bind to DNA such as histones and RNA polymerase II. Three different isoforms exist. Subcellular Location: Nucleus (By similarity). Tissue Specificity: Expressed at low levels in lung and testis. Database links: Entrez Gene: 79664 Human Entrez Gene: 93697 Mouse Omim: 610835 Human SwissProt: Q659A1 Human SwissProt: Q3UZ18 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control: U2OS.
Primary Antibody (green line): Rabbit Anti-NARG2 antibody (bs-5968R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
