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    Rabbit Anti-Shiga-like toxin IIe variant subunit A/PE-Cy5 Conjugated antibody (bs-0882R-PE-Cy5)
    訂購熱線:400-901-9800
    訂購郵箱:sales@bioss.com.cn
    訂購QQ:  400-901-9800
    技術(shù)支持:techsupport@bioss.com.cn
    說 明 書: 100ul  
    100ul/2980.00元
    大包裝/詢價(jià)
    產(chǎn)品編號(hào) bs-0882R-PE-Cy5
    英文名稱 Rabbit Anti-Shiga-like toxin IIe variant subunit A/PE-Cy5 Conjugated antibody
    中文名稱 PE-Cy5標(biāo)記的大腸桿菌志賀樣毒素Ⅱ型突變體(O139菌型)抗體
    別    名 shiga-like toxin II variant chain A precursor.  
    規(guī)格價(jià)格 100ul/2980元 購買        大包裝/詢價(jià)
    說 明 書 100ul  
    研究領(lǐng)域 細(xì)菌及病毒  
    抗體來源 Rabbit
    克隆類型 Polyclonal
    交叉反應(yīng) Escherichia Coli, 
    產(chǎn)品應(yīng)用 IF=1:50-200 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 36kDa
    性    狀 Lyophilized or Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from Escherichia coli Shiga-like toxin IIe variant subunit A
    亞    型 IgG
    純化方法 affinity purified by Protein A
    儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    產(chǎn)品介紹 background:
    Shiga-like toxin type II (SLT-II) and Shiga-like toxin type II variant (SLT-IIv) are cytotoxins produced by certain strains of Escherichia coli. Nucleotide sequence analyses had revealed that the structural genes for the A subunit and B subunit of SLT-II or SLT-IIv are arranged in an operon. Primer extension and S1 nuclease protection analyses identified a promoter for the slt-II operon 118 bases upstream of the slt-IIA gene. The slt-IIv promoter was demonstrated to be identical to the slt-II promoter. The slt-II and slt-IIv promoters differed significantly from the previously characterized Shiga toxin (stx) and Shiga-like toxin type 1 (slt-I) promoters. The transcriptional efficiencies of the stx and slt-II promoters were compared in fusions to the chloramphenicol acetyltransferase gene, and constitutive expression of the slt-II promoter was found to be equivalent to derepressed expression of the stx promoter. In contrast to the stx and slt-I promoters, the slt-II and slt-IIv promoters did not contain sequences for binding of the Fur repressor protein, and SLT-II production was not determined by iron levels in the media in various E. coli strains with wild-type or mutant ferric uptake regulation (fur) alleles. Northern (RNA) blot analysis demonstrated a single mRNA transcript for the slt-II operon, and further analysis of the slt-II operon by primer extension did not reveal an independent promoter for the B subunit gene. A putative rho-independent transcription terminator was identified 274 bases downstream of slt-IIB. These data indicated that the slt-II and slt-IIv operons differ from the stx/slt-I operon in regulation of their transcription by iron. Whether these regulatory differences enable the type I and type II groups of Shiga-like toxins to perform different roles in the pathogenesis of infectious diseases remains to be established.

    Database links:




    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    水腫病大腸桿菌志賀樣毒素Ⅱ型體,屬人、豬、羊、馬等哺乳動(dòng)物共患毒素Ⅱ型體。
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