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[KO驗證抗體] RHOC Rabbit pAb (bs-55187R)  
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50ul/1180.00元
100ul/1980.00元
大包裝/詢價

產(chǎn)品編號 bs-55187R
英文名稱 [KO驗證抗體] RHOC Rabbit pAb
中文名稱 RhoC抗體
別    名 ARH 9; ARH9; ARHC; H9; MGC1448; MGC61427; Oncogene RHO H9; Ras homolog gene family member C; RAS related homolog 9; RHO C; Rho cDNA clone 9; Rho related GTP binding protein RhoC; Rho-related GTP-binding protein RhoC; rhoC; RhoC GTPase; RHOC_HUMAN; RHOH 9;  
研究領(lǐng)域 腫瘤  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:450-100,IF=1:50-100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 22 kDa
檢測分子量
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human RHOC: 1-193/193 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Function:
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.

Subunit:
Interacts with RTKN. Interacts with AKAP13, DIAPH1, PKN2, ROCK1 and ROCK2. Interacts with ARHGDIA.

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cleavage furrow. Note=Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.

Similarity:
Belongs to the small GTPase superfamily. Rho family.

SWISS:
P08134

Gene ID:
389

Database links:

Entrez Gene: 389 Human

Entrez Gene: 295342 Rat

Omim: 165380 Human

SwissProt: P08134 Human

Unigene: 502659 Human

Unigene: 658289 Human



產(chǎn)品圖片
Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug Lane 2: RHOC knockout (KO) 293T (Human) Cell Lysate at 25 ug Primary: Anti-RHOC (bs-55187R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 22 kD Observed band size: 22 kD
Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 2: U2os (Human) Cell Lysate at 30 ug Lane 3: Huvec (Human) Cell Lysate at 30 ug Lane 4: A431 (Human) Cell Lysate at 30 ug Lane 5: U251 (Human) Cell Lysate at 30 ug Primary: Anti- RHOC (bs-55187R) at 1/1000 dilution Anti-beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD
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