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phospho-ERK1 (Thr202)+ ERK2 (Thr185) Recombinant Rabbit mAb (bsm-54491R)  
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產(chǎn)品編號 bsm-54491R
英文名稱 phospho-ERK1 (Thr202)+ ERK2 (Thr185) Recombinant Rabbit mAb
中文名稱 磷酸化絲裂原活化蛋白激酶1/2抗體
別    名 Erk1(pT202)+Erk2(pT185); Erk1(pT202)+Erk2(pT185); ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal regulated kinase  
產(chǎn)品類型 磷酸化抗體 重組兔單抗 
研究領(lǐng)域 神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  干細(xì)胞  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 6H2
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 41 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ERK1/2 around the phosphorylation site of Thr202/Thr185: FL(p-T)EY 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

SWISS:
P28482

Gene ID:
5595

Database links:

Entrez Gene: 5594 Human

Entrez Gene: 5595 Human

Entrez Gene: 26413 Mouse

Entrez Gene: 26417 Mouse

Entrez Gene: 116590 Rat

Entrez Gene: 50689 Rat

SwissProt: P27361 Human

SwissProt: P28482 Human

SwissProt: P63085 Mouse

SwissProt: Q63844 Mouse

SwissProt: P21708 Rat

SwissProt: P63086 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thyroid Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
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