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SNAP23 Recombinant Rabbit mAb (bsm-54062R)  
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50ul/1400.00元
100ul/2500.00元
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產(chǎn)品編號 bsm-54062R
英文名稱 SNAP23 Recombinant Rabbit mAb
中文名稱 突觸相關(guān)蛋白23重組兔單抗
別    名 SNAP 23; SNAP-23; SNAP23A; SNAP23B; SNP23_HUMAN; Synaptosomal associated protein 23; Synaptosomal associated protein 23kDa; Synaptosomal associated protein; Synaptosomal-associated protein 23; Vesicle membrane fusion protein SNAP 23; Vesicle membrane fusi  
研究領(lǐng)域 神經(jīng)生物學(xué)  細(xì)胞膜蛋白  
抗體來源 Rabbit
克隆類型 Recombinant
交叉反應(yīng) Human (predicted: Mouse,Chicken)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 23 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SNAP23 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

Function:
Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

Subunit:
Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3. Binds simultaneously to SNAPIN and SYN4. Found in a complex with VAMP8 and STX4 in pancreas. Interacts with STX1A and STX12 (By similarity). Binds tightly to multiple syntaxins and synaptobrevins/VAMPs. Found in a complex with VAMP8 and STX1A.

Subcellular Location:
Cell membrane. Cell membrane. Cell junction > synapse > synaptosome. Mainly localized to the plasma membrane.

Similarity:
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains.

SWISS:
O00161

Gene ID:
8773

產(chǎn)品圖片
Western blot analysis of SNAP23 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (bsm-54062R, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of SNAP23 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54062R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor?488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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