| 產(chǎn)品編號 | bs-4860R |
| 英文名稱 | Runx3 Rabbit pAb |
| 中文名稱 | Runx3抗體 |
| 別 名 | RUNX 3; RUNX-3; Runt-related transcription factor 3; Core-binding factor, alpha 3 subunit; CBF-alpha 3; Acute myeloid leukemia 2 protein; OncogeneAML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEBP2-alpha C; PEA2-alpha C; SL3-3 enhancer f |
| 研究領域 | 腫瘤 細胞生物 轉(zhuǎn)運蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Runx3: 31-130/415 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus. Function: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Subunit: Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. Subcellular Location: Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm. Post-translational modifications: Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN. Similarity: Contains 1 Runt domain. SWISS: Q13761 Gene ID: 864 Database links: Entrez Gene: 864 Human Entrez Gene: 12399 Mouse Omim: 600210 Human SwissProt: Q13761 Human SwissProt: Q64131 Mouse Unigene: 170019 Human Unigene: 378894 Mouse |
| 產(chǎn)品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Runx3 Polyclonal Antibody, Unconjugated(bs-4860R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Image provided by the Independent Validation Program, badge number 29621:A431 cells stained with Rabbit Anti-Runx3 Polyclonal Antibody at 4C overnight, followed by a Donkey Anti Rabbit FITC conjugated secondary antibody for 60 min in dark at RT
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
