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14-3-3 Tau Rabbit pAb (bs-12419R)  
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產(chǎn)品編號 bs-12419R
英文名稱 14-3-3 Tau Rabbit pAb
中文名稱 14-3-3τ蛋白抗體
別    名 1433T_HUMAN; YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; 14-3-3 Tau;  
研究領域 細胞生物  信號轉導  干細胞  細胞凋亡  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog)
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 28 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human 14-3-3 Tau: 31-130/245 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
This antibody is reactive with 14-3-3 Alpha/Beta/Gamma/Delta/Epsilon.

Subcellular Location:
Cytoplasm. In neurons, axonally transported to the nerve terminals.

Tissue Specificity:
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.

Post-translational modifications:
Ser-232 is probably phosphorylated by CK1.

Similarity:
Belongs to the 14-3-3 family.

SWISS:
P27348

Gene ID:
10971

Database links:

Entrez Gene: 10971 Human

Entrez Gene: 768311 Cow

Entrez Gene: 22630 Mouse

Entrez Gene: 25577 Rat

SwissProt: Q3SZI4 Cow

SwissProt: P27348 Human

SwissProt: P68254 Mouse

SwissProt: P68255 Rat



產(chǎn)品圖片
Sample: Liver(Rat) Cell Lysate at 40 ug Primary: Anti-14-3-3 Tau (bs-12419R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 Tau) Polyclonal Antibody, Unconjugated (bs-12419R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-14-3-3 Tau antibody (bs-12419R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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