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Trim22 Rabbit pAb (bs-12330R)  
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產(chǎn)品編號(hào) bs-12330R
英文名稱 Trim22 Rabbit pAb
中文名稱 環(huán)指蛋白94抗體
別    名 50 kDa stimulated trans acting factor; RING finger protein 94; RNF94; Staf-50; RNF94; Staf 50; STAF50; Stimulated trans acting factor(50 kDa); Stimulated Trans Acting Factor(homolog of Mouse Rpt 1 gene); Trim22; Tripartite binding motif 22; Tripartite motif containing protein 22; TTRIM22RIM22; TRI22_HUMAN.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  干細(xì)胞  淋巴細(xì)胞  t-淋巴細(xì)胞  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 57 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from Human Trim22/Staf-50/RNF94: 231-330/498 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. Staf-50 (50 kDa-stimulated trans-acting factor), also known as TRIM22 (tripartite motif-containing 22), RNF94 or GPSTAF50, is a 498 amino acid cytoplasmic protein that belongs to the TRIM family and, characteristic of TRIM family members, contains one RING-type zinc finger, one B box-type zinc finger and one SPRY domain. Induced by IFN-å and IFN-∫, Staf-50 is strongly expressed in ovary, spleen, thymus and peripheral blood leukocytes where it is thought to mediate the antiviral effects of IFN proteins. Additionally, Staf-50 is present in leukemic cells, suggesting a role in cancer formation and metastasis. Staf-50 exists as two alternatively spliced isoforms which are encoded by a gene that maps to human chromosome 11.

Function:
Trim22 is an interferon inducible protein that is preferentially expressed in cells of the haematopoietic system. Trim22 has been shown to be a p53 target gene. It also has an activation stage specific role connected to the paracrine crosstalk during T lymphocyte activation. It is strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary; expressed at basal levels in other tissues. There are two named isoforms.

Subunit:
Interacts with HIV-1 Gag polyprotein; this interaction seems to reduce gag production or virus budding. Interacts with EMCV protease 3C; this interaction leads to viral protease ubiquitination.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary. Expressed at basal levels in other tissues.

Post-translational modifications:
Auto-ubiquitinated.

Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.

SWISS:
Q8IYM9

Gene ID:
10346

Database links:

Entrez Gene: 10346 Human

Omim: 606559 Human

SwissProt: Q8IYM9 Human

Unigene: 501778 Human



產(chǎn)品圖片
Sample: Raji Cell (Human) Lysate at 40 ug U937 Cell (Human) Lysate at 40 ug Primary: Anti-Trim22 (bs-12330R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
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