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SOX7 Rabbit pAb (bs-12277R)  
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產(chǎn)品編號(hào) bs-12277R
英文名稱 SOX7 Rabbit pAb
中文名稱 轉(zhuǎn)錄因子SOX7抗體
別    名 MGC10895; SOX 7; SOX 7 transcription factor; sox7; SOX7 transcription factor; SOX7_HUMAN; SRY(sex determining region Y) box 7; SRY box 7; SRY sex determining region Y box 7; Transcription factor SOX 7; Transcription factor Sox-7; Transcription factor SOX7.  
研究領(lǐng)域 細(xì)胞生物  發(fā)育生物學(xué)  干細(xì)胞  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 42 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SOX7: 42-100/388 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Function:
Transcriptional repressor. Binds to the DNA sequence 5'-AACAAT-3'.

Subunit:
Interacts with CTNNB1/beta-catenin; this interaction may lead to the proteasomal degradation of active CTNNB1 and thus inhibition of Wnt/beta-catenin-stimulated transcription.

Subcellular Location:
Nucleus.

Tissue Specificity:
Widely expressed in adult and fetal tissues. Present both in mesenchymal and epithelial cells in some adult tissues, including colon. Tends to be down-regulated in prostate adenocarcinomas and colorectal tumors due to promoter hypermethylation.

Similarity:
Contains 1 HMG box DNA-binding domain.
Contains 1 Sox C-terminal domain.

SWISS:
Q9BT81

Gene ID:
83595

Database links:

Entrez Gene: 83595 Human

Entrez Gene: 20680 Mouse

Entrez Gene: 290317 Rat

Entrez Gene: 378665 Xenopus laevis

Omim: 612202 Human

SwissProt: Q9BT81 Human

SwissProt: P40646 Mouse

SwissProt: O42342 Xenopus laevis

Unigene: 709543 Human

Unigene: 42162 Mouse

Unigene: 1241 Xenopus laevis



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX7) Polyclonal Antibody, Unconjugated (bs-12277R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX7) Polyclonal Antibody, Unconjugated (bs-12277R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SOX7 Polyclonal Antibody, Unconjugated(bs-12277R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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