| 產(chǎn)品編號 | bs-11201R |
| 英文名稱 | Musashi 1 Rabbit pAb |
| 中文名稱 | 神經(jīng)干細(xì)胞RNA結(jié)合蛋白Musashi1抗體 |
| 別 名 | Musashi 1/Msi1; Msi 1; Msi1; Msi-1; MSI1H_HUMAN; Musashi homolog 1; Musashi-1; Musashi1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1. |
| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 干細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Musashi 1: 66-150/362 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary. Function: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. Similarity: Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains. SWISS: O43347 Gene ID: 4440 Database links: Entrez Gene: 4440 Human Entrez Gene: 17690 Mouse Omim: 603328 Human SwissProt: O43347 Human SwissProt: Q61474 Mouse Unigene: 158311 Human Unigene: 5077 Mouse Unigene: 162146 Rat Musashi1是一種進(jìn)化保守的RNA結(jié)合蛋白，在維持干細(xì)胞狀態(tài)、分化和腫瘤發(fā)生方面起著重要作用。Musashi1 選擇性地表達(dá)在神經(jīng)前體細(xì)胞上,包括神經(jīng)干細(xì)胞上。在神經(jīng)系統(tǒng)外，Musashi1還是腸干細(xì)胞的選擇性標(biāo)記。這些組織干細(xì)胞或未成熟細(xì)胞Musashi1的表達(dá)，表明Musashi1在轉(zhuǎn)錄后基因調(diào)節(jié)階段維持這些細(xì)胞未分化狀態(tài)起重要作用。Musashi1過度表達(dá)通過依賴RBP2Jk的旁路激活Notch1，而Notch信號途徑功能為誘導(dǎo)哺乳動物神經(jīng)干細(xì)胞自我更新。通過musashi1-P-小鼠培養(yǎng)腦細(xì)胞的Musashi蛋白產(chǎn)物反義去除研究,發(fā)現(xiàn)這些基因在維持神經(jīng)干細(xì)胞未分化狀態(tài)起著重要的作用。 Musashi抑制m-Numb轉(zhuǎn)錄的分子機制尚待進(jìn)一步研究。Musashi1有可能除轉(zhuǎn)錄調(diào)控外還參與其他調(diào)控途徑。另外，Musashi1還表達(dá)在一些腦腫瘤的特殊類型(這些腫瘤可能起源自非成熟腦細(xì)胞)，并且表達(dá)水平和腫瘤的惡性程度及增殖能力相關(guān)。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Musashi 1) Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Musashi-1 Polyclonal Antibody, Unconjugated(bs-11201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Musashi-1 Polyclonal Antibody, Unconjugated(bs-11201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Musashi 1) polyclonal Antibody, Unconjugated (bs-11201R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Musashi 1 antibody (bs-11201R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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