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KIF11 Rabbit pAb (bs-8177R)  
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產(chǎn)品編號 bs-8177R
英文名稱 KIF11 Rabbit pAb
中文名稱 驅(qū)動蛋白樣蛋白1/甲狀腺激素受體相互作用蛋白5抗體
別    名 HKSP; kinesin family member 11; Kinesin like protein 1; Kinesin like spindle protein HKSP; KNSL1; Eg5; Thyroid receptor interacting protein 5; TRIP5; KIF11_HUMAN.  
Specific References  (1)     |     bs-8177R has been referenced in 1 publications.
[IF=4.86] Junjun Ling. et al. KIF11, a plus end-directed kinesin, as a key gene in benzo(a)pyrene-induced non-small cell lung cancer. Environ Toxicol Phar. 2022 Jan;89:103775  IHC ;  Human.  
研究領(lǐng)域 細胞生物  信號轉(zhuǎn)導(dǎo)  細胞周期蛋白  細胞骨架  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 120 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human KIF11/Eg5: 351-450/1056 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Eg5 is a motor protein that belongs to the kinesin-like family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

Function:
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Subunit:
Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis.

Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton, spindle pole.

Post-translational modifications:
Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

Similarity:
Belongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain.

SWISS:
P52732

Gene ID:
3832

Database links:

Entrez Gene: 3832 Human

Omim: 148760 Human

SwissProt: P52732 Human

Unigene: 8878 Human



產(chǎn)品圖片
Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-KIF11 (bs-8177R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD
Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KIF11 Polyclonal Antibody, Unconjugated(bs-8177R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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