| 產(chǎn)品編號 | bs-4253R |
| 英文名稱 | SMAD9 Rabbit pAb |
| 中文名稱 | SMAD家族9抗體 |
| 別 名 | AI528653; BSP 1; BSP1; DKFZp781C1895; DKFZp781O1323; Dwarfin A; Dwf A; DwfA; Dwfc; hSMAD1; hSmad5; JV 41; JV4 1; JV41; JV5 1; MAD(mothers against decapentaplegic, Drosophila) homolog 1; MAD(mothers against decapentaplegic, Drosophila) homolog 9; MAD homol |
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Specific References (2) | bs-4253R has been referenced in 2 publications.
[IF=4.427] Lijie Dong. et al. RNA sequencing reveals BMP4 as a basis for the dual-target treatment of diabetic retinopathy. J Mol Med. 2021 Feb;99(2):225-240 WB,IF ; Monkey, Rat, Human.
[IF=3.15] Yang Zhai. et al. Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma. Bmc Cancer. 2021 Dec;21(1):1-13 IHC ; Human.
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| 研究領域 | 腫瘤 免疫學 染色質(zhì)和核信號 信號轉(zhuǎn)導 干細胞 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SMAD9: 361-467/467 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). Subunit: Interaction with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit. Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Note=In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity). Tissue Specificity: Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney. Post-translational modifications: Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. DISEASE: Defects in SMAD9 may be a cause of primary pulmonary hypertension (PPH1) [MIM:178600]. A rare disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs. Similarity: Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain. SWISS: O15198 Gene ID: 4093 Database links: Entrez Gene: 4093 Human Entrez Gene: 55994 Mouse Omim: 603295 Human SwissProt: O15198 Human SwissProt: Q9JIW5 Mouse Unigene: 123119 Human Unigene: 244353 Mouse Unigene: 10862 Rat |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-SMAD9 (bs-4253R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Skin tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human A549 cell lysates
Lane 6: Human HepG2 cell lysates
Primary: Anti-SMAD9 (bs-4253R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD9) Polyclonal Antibody, Unconjugated (bs-4253R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Positive control: Hela cells
Concebtration: 3μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
