| 產(chǎn)品編號 | bs-1117R |
| 英文名稱 | GPR49 Rabbit pAb |
| 中文名稱 | G蛋白偶聯(lián)受體49抗體 |
| 別 名 | LGR5; G protein coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; GPCR GPR49; G protein coupled receptor 67; FEX; g protein-coupled receptor fex; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; Leucine rich |
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Specific References (3) | bs-1117R has been referenced in 3 publications.
[IF=17.373] Lai Wei. et al. Serotonin Deficiency is Associated with Delayed Gastric Emptying. Gastroenterology. 2021 Mar;: IHC ; Human.
[IF=3.337] Maiko Machida. et al. Methotrexate mediates the integrity of intestinal stem cells partly through nitric oxide-dependent Wnt/β-catenin signaling in methotrexate-induced rat ileal mucositis. J Pharmacol Sci. 2022 Mar;148:281 IHC ; Rat.
[IF=0] Hosomichi J et al. Localization of leucine-rich repeat-containing G-protein-coupled receptor 5- and Ki67-positive periodontal cells expressing runt-related transcription factor 2 during tooth movement. Orthodontic Waves.2018. IHF&WB ; Rat&Human.
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| 研究領域 | 腫瘤 信號轉(zhuǎn)導 干細胞 細胞凋亡 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 98 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse GPR49: 810-907/907 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Function: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. Subunit: Identified in a complex composed of RNF43, LGR5 and RSPO1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. Similarity: Belongs to the G-protein coupled receptor 1 family. Contains 16 LRR (leucine-rich) repeats. Contains 1 LRRNT domain. SWISS: Q9Z1P4 Gene ID: 14160 Database links: Entrez Gene: 8549 Human Entrez Gene: 14160 Mouse Omim: 606667 Human SwissProt: O75473 Human SwissProt: Q9Z1P4 Mouse Unigene: 658889 Human Unigene: 42103 Mouse Unigene: 214063 Rat Lgr5基因（Wnt細胞信號系統(tǒng)）是一種G蛋白偶聯(lián)受體，已經(jīng)被確定為幾種成年組織和癌癥中干細胞的一個獨特標記。Lgr5最初是在結(jié)腸癌細胞中發(fā)現(xiàn)的，有報道稱：在惡化前的小鼠腺瘤中也有發(fā)現(xiàn)，這說明Lgr5很可能也是其他組織成體干細胞和癌癥干細胞的標志物。 |
| 產(chǎn)品圖片 |
Sample:
LOVO(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-GPR49 (bs-1117R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 125 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-GPR49 (bs-1117R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 98 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (bs-1117R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR49/LGR5 Polyclonal Antibody, Unconjugated(bs-1117R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR49/LGR5 Polyclonal Antibody, Unconjugated(bs-1117R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line):Hela(blue).
Primary Antibody (green line): Rabbit Anti-GPR49 antibody(bs-1117R),
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
